A Machine-Learning Approach Identifies Rejuvenating Interventions in the Human Brain

MicroRNA-mediated neuronal detargeting alters astrocyte cell fate conversion trajectories in vivo

• Mutated Ascl1SA6 and Ascl1SA6 -124T convert astrocytes to neuron-like cells in lineage tracing model .
• Converted astrocytes are enriched in S/G2-M cell cycle phases and have low GSK3 signaling.
• miR-124T sequences detarget transgene expression in endogenous neurons and alter the fate trajectory of reprogrammed astrocytes.
• miR-124T sequences diversify lineages, biasing astrocytes towards a glutamatergic rather than GABAergic neuronal fate and inducing an oligodendrocyte fate.

Regenerative Medicine and the Bioethics of Globalization, Justice and Hegemony

Regenerative Medicine Innovation and Governance: Insights from the Global South

Making Advanced Therapies Affordable and Accessible: Two Strategic Approaches

• Current problem with gene therapies: Advanced therapies that are scientifically validated are not reaching patients due to accessibility and affordability valleys of death.
• Rehabilitative interventions improve pricing, streamline regulation, enhance manufacturing, and support payer engagement
• Transformative interventions centre access and affordability from the outset of the research and development process, including changing how intellectual property is governed, how public investment is translated into public benefit, and how product profiles are aligned with real-world conditions, particularly in low-resource settings.
• The future of advanced therapies depend on both technological progress and on the willingness to adopt policies and practices that treat affordability and access as central concerns

Report on Regulatory Frameworks for Clinical Research on and Marketing Authorization of Regenerative Medicine Products in Low- and Middle-Income Countries

Report on Regenerative Medicine Clinical Research Activities and Product Pipelines in Low- and Middle- Income Countries in Africa and Asia

Efficient DNA- and virus-free engineering of cellular transcriptomic states using dCas9 ribonucleoprotein (dRNP) complexes

Regionally distinct GFAP promoter expression plays a role in off-target neuron expression following AAV5 transduction

Comparing Viral Vectors and Fate Mapping Approaches for Astrocyte-to-Neuron Reprogramming in the Injured Mouse Cerebral Cortex

An E. coli-based platform for the production and assembly of anellovirus vectors

The chromatin remodeler ADNP regulates neurodevelopmental disorder risk genes and neocortical neurogenesis

Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1

Alphaviral backbone of self-amplifying RNA enhances protein expression and immunogenicity against SARS-CoV-2 antigen

Next Generation Aqueous Two-Phase System for Gentle, Effective, and Timely Extracellular Vesicle Isolation and Transcriptomic Analysis

Examining the NEUROG2 lineage and associated gene expression in human cortical organoids

Highlights

• NEUROG1 and NEUROG2 are most highly expressed in basal neural progenitor cells, NEUROG1-derived lineages predominate early and NEUROG2 lineages later.
• NEUROG2 is necessary and sufficient to directly transactivate known target genes (NEUROD1, EOMES, RND2)
• Neurog2 similarly induces Col3a1 & Ppp1r17 in murine P19 cells, consistent with a conservation of NEUROG2 function across mammalian species.

Detecting expressed genes in cell populations at the single-cell level with scGeneXpress

Highlights

• scGeneXpress: a new computational tool designed for identifying expressed genes in single-cell RNA sequencing data.
• scGeneXpress improves the detection of genes expressed at low levels, allowing for a more precise understanding of cellular functions.
• This tool is useful for research in complex tissues, where distinguishing gene expression among different cell types is essential.
• The tool's design is accessible, making it a practical option for researchers without extensive computational expertise.
• This computational tool is relevant for cell engineering, since it enables a more accurate identification of the identity transcription factors of cell populations, which will be used for cellular conversion.

Direct Neuronal Reprogramming of NDUFS4 patient cells identifies the unfolded protein response as a novel general reprogramming hurdle

Highlights

• NDUFS4-mutant patient astrocytes are impaired in reprogramming into neurons
• Unfolded protein response inhibition improves neuronal reprogramming
• Patient iNs are affected in neuronal subtype specification
• Transient unfolded protein inhibition leads to more functional iNs

The ChAHP chromatin remodelling comple regulates neurodevelopmental disorder risk genes to scale the production of neocortical layers

Highlights

• Adnp and Chd4 cKOs exhibit similar deficits in cortical growth
• Adnp sustains the proliferation of apical progenitors to scale the production of upper-layer neurons
• Adnp recruits Chd4 to genes involved in corticogenesis
• Adnp is a master regulator of risk genes associated with neurodevelopmental disorders